Isolation and characterization of polymorphic microsatellite DNA markers in the Japanese eight-barbel loach, Lefua echigonia

摘要

We isolated and characterized 17 polymorphic microsatellite loci for the Japanese eight-barbel loach, Lefua echigonia, an endangered freshwater species in streams including agricultural canals in Japan. The number of observed alleles for each locus ranged from two to nine, and the values of observed and expected heterozygosities ranged from 0.125 to 0.844 and from 0.148 to 0.876, respectively. All loci conformed to Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.