Identification of microsatellite loci from Epimedium koreanum and cross-species amplification in four species of Epimedium (Berberidaceae)

摘要

Nineteen polymorphic microsatellite markers were isolated and characterized from a trinucleotide enriched partial genomic library of Epimedium koreanum. The average allele number of these microsatellites was 5.9 per locus, ranging from two to 11. The observed heterozygosity (H-O) and expected heterozygosity (H-E) at the population level were 0.00-0.90 and 0.12-0.90, respectively. In addition, the results of cross-species amplification of this set of microsatellite markers in four closely related Epimedium species, E. brevicornum, E. sagittatum, E. pubescens and E. wushanense, revealed that these microsatellite markers were useful for population genetic structure evaluation and genotype analysis of major Epimedium species that have been used as traditional Chinese medicines.