Thirty-two single nucleotide polymorphism markers for high-throughput genotyping of sockeye salmon

摘要

We characterize 32 single nucleotide polymorphism genotyping assays for resolving genotypic variation in sockeye salmon Oncorhynchus nerka in the Pacific Rim. These assays are based on the 5'-nuclease reaction and thus facilitate high-throughput genotyping with minimal optimization time. Minor allele frequency differences (Delta q) among collections were between 4.7% and 97.9%, resulting in per locus F-ST estimates of 0.02-0.71 with an average of 0.22.