Isolation and characterization of microsatellite loci in a marine fish species, the tusk (Brosme brosme)

摘要

We developed polymerase chain reaction primers for nine dinucleotide microsatellite loci in the marine deep-sea fish, the tusk (Brosme brosme). All markers were obtained from a partial genomic DNA library, and characterized in 100 unrelated individuals from one putative population. The number of alleles ranged from two to 60 with an average of 15/locus. The observed heterozygosity ranged from 0.020 to 0.826 (average 0.438). Several of the markers amplified multiple alleles from the two other gadoid species tested.