New microsatellite loci for the European bushcricket, Ephippiger ephippiger (Orthoptera : Tettigoniidae)

摘要

Ephippiger ephippiger is a model organism for studies of sexual selection and phylogeography but little is known about fine-scale population structure. Available microsatellite loci have null allele problems so we used an enrichment technique to isolate 21 new microsatellite loci for E. ephippiger. We present primer pairs for 10 polymorphic loci (3-11 alleles per locus). Observed heterozygosities at polymorphic loci ranged from 0.118 to 0.787, but several were significantly lower than expected.