Isolation of polymorphic microsatellite loci in the genus Clusia (Clusiaceae)

摘要

Microsatellite flanking region sequences may provide phylogenetically useful information. We isolated 13 polymorphic microsatellite loci from two species, Clusia minor (five loci) and Clusia nemorosa (eight loci), to aid in the determination of phylogenetic relationships within the genus Clusia. Eleven loci amplified across all 17 Clusia species tested, while two loci amplified in 10 out of 17 species. The extensive cross-species amplification suggests that these loci may be useful for an examination of phylogenetic relationships in this genus.