Ten microsatellite loci for evolutionary research on Nesospiza buntings

摘要

We tested 19 loci originally developed for Vidua and Geospiza, of which six Vidua loci were polymorphic and furthermore, developed four microsatellite loci for Nesospiza buntings. Allelic variation ranged from two to 13 alleles, with heterozygosity from 0 to 0.82. Ascertainment bias was evident as increased allelic diversity of loci developed for Nesospiza, versus those developed for Vidua. Two loci showed linkage, and several, deviation from Hardy-Weinberg equilibrium. We suggest such deviations are through introduction of new alleles by introgression, or of the Wahlund effect. These loci will contribute to the understanding of speciation in Nesospiza buntings of the Tristan da Cunha islands.