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首页> 外文期刊>Molecular ecology notes >Characterization of microsatellite loci in the aphid species Macrosiphoniella tanacetaria (Homoptera, Aphididae)
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Characterization of microsatellite loci in the aphid species Macrosiphoniella tanacetaria (Homoptera, Aphididae)

机译:蚜虫种乙酰丙酮尿杆菌(Honoptera,Aphididae)中微卫星基因座的特征

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摘要

Measuring dispersal in small insects can now be achieved through the development of highly polymorphic molecular markers. We isolated eight microsatellites in the aphid species Macrosiphoniella tanacetaria Kaltenbach, feeding on the common Tansy (Tanacetum vulgare L.). For each locus, we observed between three and seven alleles and expected heterozogosities ranged between 0.29 and 0.76 within 45 individuals (five aphids/population * nine populations = 45). First analyses revealed that all individuals had different genotypes, showing a great potential variability within and between populations, facilitating the further study of dispersal in aphids.
机译:现在,可以通过开发高度多态的分子标记物来测量在小昆虫中的扩散。我们以普通的艾菊(艾菊)为食,分离了蚜虫类拟南芥Kaltenbach蚜虫中的八个微卫星。对于每个基因座,我们观察到45个个体(5个蚜虫/种群* 9个种群= 45)中有3至7个等位基因,预期的杂合度在0.29至0.76之间。初步分析显示,所有个体都有不同的基因型,显示出种群内部和种群之间存在巨大的潜在变异性,这有助于进一步研究蚜虫的传播。

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