Universal primers for a large cryptically simple cpDNA microsatellite region in Aristolochia (Aristolochiaceae)

摘要

We provide a new and valuable marker to study species relationships and population genetics in order to trace evolutionary, ecological, and conservational aspects in the genus Aristolochia. Universal primers for amplification and subsequent sequencing of a chloroplast microsatellite locus inside the trnK intron are presented. Utility of the primers has been tested in 32 species representing all clades of Aristolochia, including population studies within the A. pallida complex, A. clusii and A. rotunda. The microsatellite region is characterized as a (A(n)T(m))(k) repeat of 22-438 bp containing a combination of different repeats arranged as 'cryptically simple'.