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首页> 外文期刊>Molecular medicine reports >A Chinese herbal decoction can increase the intracellular Ca2+ concentration and CatSper1 expression in mouse sperm tails
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A Chinese herbal decoction can increase the intracellular Ca2+ concentration and CatSper1 expression in mouse sperm tails

机译：中草药汤可提高小鼠精子尾巴中细胞内Ca2 +浓度和CatSper1表达

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The aim of this study was to investigate the impact of a Chinese herbal decoction on the intracellular calcium (Ca2+) concentration of sperm and the expression of the cation channel 1 of sperm (CatSper1), which is a calcium-channel protein specific to sperm tail, in a murine model of asthenospermia induced with cyclophosphamide. After 34 days of intragastric administration of Chinese herb decoction to the murine model used, routine analyses of the mouse sperm were conducted, the intracellular Ca2+ concentration of the sperm tails was measured using flow cytometry, and the expression of CatSper1 protein was detected using reverse transcription-polymerase chain reaction (RT-PCR). Sperm concentration, percentage of grade A and B sperm (i.e., sperm activity) and percentage of grade A, B and C sperm (i.e., overall sperm motility) of the model group mice (MG) were markedly lower compared to the control murine group (CG) (one-way ANOVA, P<0.05). Subsequent to treatment, sperm concentration, percentage of sperm activity and overall sperm motility of the large dose of herbal medicine group murine were markedly increased compared to MG mice (P<0.05). Intracellular Ca2+ concentration in MG mice was markedly lower compared to CG mice (P<0.05). However, following therapy, a significant increase was observed in the intracellular Ca2+ concentration in LG mice as compared to MG mice (P<0.05). In addition, the expression of CatSper1 in LG mice was significantly higher compared to MG mice (P<0.05), while no statistically significant difference was observed for the CG mice. Intraperitoneal injection of cyclophosphamide reduced sperm concentration, percentage of sperm activity and overall sperm motility, intracellular Ca2+ concentration and CatSper1 expression. Large doses of this Chinese herbal decoction increased sperm intracellular Ca2+ concentration, sperm concentration, and percentages of sperm activity and overall sperm motility by upregulating CatSper1 expression. The findings of this study. have demonstrated a therapeutic effect of this decoction on asthenospermia.
机译：这项研究的目的是研究中药汤对精子细胞内钙（Ca2 +）浓度和精子阳离子通道1（CatSper1）的表达的影响，CatSper1是精子尾巴特有的钙通道蛋白在环磷酰胺诱导的小鼠弱精子症模型中。对所用小鼠模型进行胃内灌胃中药汤后第34天，进行小鼠精子的常规分析，使用流式细胞仪检测精子尾巴的细胞内Ca 2+浓度，并通过逆转录检测CatSper1蛋白的表达。 -聚合酶链反应（RT-PCR）。与对照组相比，模型组小鼠（MG）的精子浓度，A级和B级精子百分比（即精子活性）以及A，B和C级精子百分比（即总体精子活力）明显较低。 （CG）（单向方差分析，P <0.05）。与MG小鼠相比，大剂量草药组鼠的治疗后，精子浓度，精子活性百分比和总体精子运动性显着提高（P <0.05）。与CG小鼠相比，MG小鼠的细胞内Ca2 +浓度显着降低（P <0.05）。但是，治疗后，与MG小鼠相比，LG小鼠的细胞内Ca2 +浓度显着增加（P <0.05）。此外，与MG小鼠相比，LG小鼠中CatSper1的表达显着更高（P <0.05），而CG小鼠未观察到统计学上的显着差异。腹腔注射环磷酰胺可降低精子浓度，精子活性和总精子活力百分比，细胞内Ca2 +浓度和CatSper1表达。通过上调CatSper1表达，大剂量这种中草药汤可提高精子细胞内Ca2 +浓度，精子浓度以及精子活性和总体精子活力的百分比。这项研究的结果。已经证明了该汤对弱精症的治疗作用。

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