An analysis of images of opaque objects in the problem of the optimization of the focusing of optical systems

摘要

An urgent problem of the optimization of automated optical microscopy is considered. An approach is suggested during which variations in the brightness of a Gaussian beam in microscope optics are analyzed. A discrete (digital) model, which correlates with the continuous one, is introduced. An analytical approach to finding the maximum of a discrete function that estimates the measure of optical system focusing is suggested; it is based on a discrete model of Gaussian beam behavior and requires a much smaller number of optical microscope images, which speeds up the process of the automated focusing of an optical microscope.