Gua Lou Gui Zhi decoction attenuates post-stroke spasticity via the modulation of GABA(B) receptors

摘要

The aim of the present study was to investigate the mechanisms underlying the neuroprotective and antispastic effects of Gua Lou Gui Zhi decoction (GLGZD) in a rat model of middle cerebral artery occlusion (MCAO). The MCAO rats were treated with GLGZD (14.3 g/kg body weight) once a day for a period of seven days. Neurological deficit scores and screen tests were analyzed every other day. Following treatment with GLGZD for 7 days, the ischemic infarct volume of the rat brains was measured using 2,3,5-triphenyl tetrazolium chloride staining. Reverse transcription-polymerase chain reaction was performed in order to determine the mRNA expression levels of gamma-amino butyric acid B (gamma-GABA(B)) receptor (R) in the cortical infarct region. Furthermore, the protein expression levels of GABA(B) R were detected in the cortical infarct region by western blot analysis. Following 7 days, treatment with GLGZD significantly ameliorated the neurological defects and cerebral infarction in the MCAO rats. In addition, treatment with GLGZD ameliorated motor performance in the MCAO rats, as determined by screen tests. Furthermore, GLGZD was able to upregulate the mRNA and protein expression levels of GABA(B1) R and GABA(B2) R in the ischemic cerebral cortex. The results of the present study suggested that GLGZD may exert neuroprotective and antispastic effects in a cerebral ischemia model, through upregulating the expression of GABA(B) R.