Correlation between conventional sperm assay parameters in cryopreserved Ram Semen

摘要

This study investigated the correlation of cryopreserved sperm membrane integrity with viability and motility in ram semen diluted in five different extenders and storage in LN (Liquid Nitrogen). Semen was collected with an artificial vagina from five adult rams. The ejaculates were pooled and diluted in five different extenders: Milk, Sodium citrate, Tris, Lactose and Sucrose. The diluted semen was frozen in vapor of LN (Liquid Nitrogen) and stored in LN.Afterthawed, sperm viability, progressive motility and membrane integrity was evaluated. Statistical analysis of the data revealed a significant (p0.01) positive correlation between viability, progressive motility and membrane integrity in cryopreserved ram semen. Therefore, these results have indicated that there are statisticallysignificant correlations between evaluated parameters. It was concluded that semen characteristics could be evaluated by each of this parameters instead of evaluation all of them, accompanied with other assays for use in fertilization in vivo and in vitro.