Development and cross-species amplification of microsatellite markers from the endangered Wee Jasper Grevillea (Grevillea iaspicula, Proteaceae)

摘要

Grevillea iaspicula is a highly restricted species occupying limestone outcrops near Wee Jasper, New South Wales (Australia). To allow future assessment of the ecological processes shaping the structure of G. iaspicula populations, seven polymorphic microsatellite (SSR) loci were developed from a partial genomic library. Amplification and polymorphism of six previously derived G. macleayana SSR primer pairs in the target species, G. iaspicula, were also assessed and cross-species amplification of SSR primers among several grevillea species is discussed.