Metabolomics study on model rats of chronic obstructive pulmonary disease treated with Bu-Fei Jian-Pi

摘要

The therapeutic effect of Traditional Chinese Medicine (TCM) on chronic obstructive pulmonary disease (COPD) has been know for numerous years; however, the mechanism of action of the beneficial effects of TCM remains to be elucidated. The present study aimed to investigate the molecular mechanisms of COPD through metabolomic analysis as well as explore the targets and intervention mechanisms of TCM therapy using the common TCM granules Bu-Fei Jian-Pi. COPD rat models were established using smoke inhalations and recurrent bacterial infections. Rats were then divided into three groups as follows: A1, control healthy rats; B1, COPD model; and D1, Bu-Fei Jian-Pi-treated COPD rats. Following administration of the medicine, the metabolomic profile of the lung tissue of rats in each group was assessed using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry. The results demonstrated that there was a significanlty different spectrum of metabolites in the lung tissue of the model group compared to that of the control group as well as the Bu-Fei Jian-Pi-treated COPD group; in addition, following treatment with Bu-Fei Jian-Pi, the metabolites of COPD rats were comparable with those of the control. Notable changes were observed in 31 metabolites between the Bu-Fei Jian-Pi-treated group and the model group; however, there were 13 comparable metabolites between the Bu-Fei Jian-Pi and control groups as well as the model and control groups. Eleven metabolites showed a negative fold change in the Bu-Fei Jian-Pi-treated groups compared to concentrations in the model group; however, minimal changes were observed in phenylpyruvic acid and alpha-D-fucose expression. In conclusion, the results of the present study demonstrated that Bu-Fei Jian-Pi granules had beneficial effects on measured outcomes in a rat model of stable COPD, indicated by a significantly different spectrum of metabolites. This therefore indicated that the metabolites which had significantly altered expression in the model group compared with that of the control and Bu-Fei Jian-Pi-treated groups may be potential biomarkers of COPD.