Characterization of a set of common wheat-Hordeum chilense chromosome 7H(ch) introgression lines and its potential use in research on grain quality traits

摘要

Chromosome 7H(ch) from Hordeum chilense has potential for improving seed carotenoid content in wheat as it carries a Phytoene synthase 1 (Psy1) gene, which has a major role in this trait. Structural changes in chromosome 7H(ch) were obtained in common wheat background by crossing the wheat disomic substitution line 7H(ch)(7D) with a disomic addition line carrying chromosome 2C(c) from Aegilops cylindrica in common wheat cv. Chinese Spring'. Rearranged 7H(ch) chromosomes were cytologically characterized by FISH. A set of 24 molecular markers and the Psy1 gene were used to identify the H.chilense chromosome segments involved in the introgressions. Six structural rearrangements of chromosome 7H(ch) were identified. They included three homozygous wheat-H.chilense centromeric translocations, one involving the 7H(ch)S arm (T-7H(ch)SA/B) and two involving the 7H(ch)L arm (T1-7H(ch)LA/B and T2-7H(ch)LA/B). In addition, one 7H(ch)S arm deletion, one 7H(ch)L7H(ch)L isochromosome and one 7H(ch)S telosome were obtained in hemizygous condition. These genetic stocks will be useful for studying the effect of chromosome 7H(ch) on wheat flour colour.