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Gene expression profiling of Sinapis alba leaves under drought stress and rewatering growth conditions with Illumina deep sequencing

机译:利用Illumina深度测序研究干旱和复水生长条件下剑叶芥叶片的基因表达。

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Sinapis alba has many desirable agronomic traits including tolerance to drought. In this investigation, we performed the genome-wide transcriptional profiling of S. alba leaves under drought stress and rewatering growth conditions in an attempt to identify candidate genes involved in drought tolerance, using the Illumina deep sequencing technology. The comparative analysis revealed numerous changes in gene expression level attributable to the drought stress, which resulted in the down-regulation of 309 genes and the up-regulation of 248 genes. Gene ontology analysis revealed that the differentially expressed genes were mainly involved in cell division and catalytic and metabolic processes. Our results provide useful information for further analyses of the drought stress tolerance in Sinapis, and will facilitate molecular breeding for Brassica crop plants.
机译:芥末(Sinapis alba)具有许多理想的农艺性状,包括对干旱的耐受性。在这项研究中,我们使用Illumina深度测序技术,在干旱胁迫和补水生长条件下对白叶念珠菌叶片进行了全基因组转录谱分析,以试图鉴定参与耐旱性的候选基因。比较分析表明,干旱胁迫导致基因表达水平发生了许多变化,导致309个基因的下调和248个基因的上调。基因本体分析表明,差异表达的基因主要参与细胞分裂以及催化和代谢过程。我们的结果为进一步分析Sinapis的干旱胁迫提供了有用的信息,并将有助于芸苔属作物植物的分子育种。

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