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首页> 外文期刊>Molecular biology of the cell >Association of prenylated proteins with the plasma membrane and the inner nuclear membrane is mediated by the same membrane-targeting motifs
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Association of prenylated proteins with the plasma membrane and the inner nuclear membrane is mediated by the same membrane-targeting motifs

机译:异戊二烯基化蛋白与质膜和内核膜的缔合由相同的靶向膜的基序介导

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摘要

Targeting of nuclear lamins to the inner nuclear envelope membrane requires a nuclear localization signal and CaaX motif-dependent posttranslational modifications, including isoprenylation and carboxyl methylation. These modifications, although necessary for membrane targeting, are not sufficient to mediate stable association with membranes. We show that two variants of lamin B3 (i.e., B3a and B3b) exist in Xenopus oocytes. They are encoded by two alternatively spliced, developmentally regulated mRNAs. The two lamin variants differ greatly in their membrane association in meiotically matured eggs. The presence of an extra cysteine residue (as a potential palmitoylation site) and a basic cluster in conjunction with the CaaX motif function as secondary targeting signals responsible for the stable membrane association of lamin B3b in Xenopus eggs. Moreover, transfection experiments with Green Fluorescent Protein lamin tail chimeras and with a Green Fluorescent Protein N-Ras chimera show that these secondary motifs are sufficient to target proteins to the inner nuclear membrane and/or the plasma membrane. Implications for the intracellular trafficking of doubly lipidated proteins are discussed. [References: 62]
机译:将核纤层蛋白靶向内核被膜需要核定位信号和依赖于CaaX基序的翻译后修饰,包括异戊二烯基化和羧基甲基化。这些修饰尽管对于膜靶向是必需的,但不足以介导与膜的稳定缔合。我们显示非洲爪蟾B3(即B3a和B3b)的两个变体存在于非洲爪蟾卵母细胞中。它们由两个选择性剪​​接的,发育调控的mRNA编码。在减数分裂成熟的卵中,这两种核纤层蛋白变体的膜结合差异很大。额外的半胱氨酸残基(作为潜在的棕榈酰化位点)和与CaaX基序结合的基本簇的存在,是次生靶向信号,负责非洲爪蟾卵中lamin B3b的稳定膜缔合。而且,用绿色荧光蛋白核纤层蛋白嵌合体和绿色荧光蛋白N-Ras嵌合体的转染实验表明,这些次级基序足以将蛋白靶向内核膜和/或质膜。讨论了双脂化蛋白在细胞内运输的意义。 [参考:62]

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