Identification of reproduction-related proteins and characterization of proteasome alpha 3 and proteasome beta 6 cDNAs in testes of the giant tiger shrimp Penaeus monodon

摘要

Cellular proteomic analysis was carried out to identify reproduction-related proteins in testes of wild and domesticated broodstock of Penaeus monodon. In total, 642 protein spots were characterized and 287 spots (44.70%) significantly matched protein sequences in the databases (P<0.05). To examine a role of the proteasome system in testicular development of P. monodon, the expression profiles of proteasome alpha 3 subunit (PmPsma3) and proteasome beta 6 (PmPsmb6) mRNA in different groups of domesticated shrimp and in wild broodstock were examined. The expression levels of these transcripts in testes of 18-month-old domesticated shrimp were significantly lower than those of wild broodstock (P<0.05). Interestingly, the expression levels of testicular PmPsma3 and PmPsmb6 in 18-month-old shrimp were significantly increased at 24. h following serotonin injection (50. μg/g body weight). Results suggested that reduced degrees of maturation in captive P. monodon males may be partially resolved by exogenous 5-HT administration.