Relations between macro- and microstability of C(H)2 domains of human IgG2 and their biological activity: 2. Calculation of thermodynamic functions that characterize the domain stability

摘要

Thermodynamic parameters of stabilization of the native structure of C(H)2 domains in human myeloma second subclass LOM and SIN immunoglobulins and their hFc fragments, which we obtained for the first time, were determined using the method of scanning microcalorimetry. The decrease in thermal stability and energy of stabilization of the native structure of the domains in intact proteins in the physiological range of temperatures (20-37A degrees C) correlated mainly with the entropy factor.