Development of Hybrid Human Interferon Alfa-2 Strain-Producers and the Use of Enteropeptldase for Production of N-Terminal Methionine-Free Interferons

摘要

We developed a technique for production of human interferon-α2a (IFN-α2a) and IFN-α2b lacking the N-terminal methionine. First of all, we constructed plasmids containing genes of hybrid IFN-α2 controlled by different promoters; the proximal part of the genes incorporated various sequences encoding the enteropeptidase cleavage site. As a result, four strains of Escherichia coli producing hybrid IFN-α2 were obtained. The protocol for IFN-α2 renaturation, cleavege of its N-terminal part, and chromatographic purification of the N-terminal methionine-free IFN-α2 was developed.