Rapid detection of alpha-thalassaemia alleles of (_SEA)/, -alpha(3.7)/ and -alpha(4.2)/ using a dual labelling, self-quenching hybridization probe/melting curve analysis

Gao Lan; Liu Yanhui; Sun Manna; Zhao Ying; Xie Rungui; He Yi; Xu Wanfang; Liu Jianxin; Lin Yangyang; Lou Jiwu

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Rapid detection of alpha-thalassaemia alleles of (_SEA)/, -alpha(3.7)/ and -alpha(4.2)/ using a dual labelling, self-quenching hybridization probe/melting curve analysis

机译：使用双重标记，自猝灭杂交探针/熔解曲线分析快速检测（_SEA）/，-α（3.7）/和-alpha（4.2）/的α地中海贫血等位基因

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摘要

Objectives: The aim of the study was to set up an alternative automatic molecular diagnostic method for deletional alpha-thalassaemia mutations without gel electrophoresis.

机译：目的：本研究的目的是建立一种无需凝胶电泳即可替代的α-地中海贫血突变的自动分子诊断方法。

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《Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines》 |2015年第6期|共4页
作者
Gao Lan; Liu Yanhui; Sun Manna; Zhao Ying; Xie Rungui; He Yi; Xu Wanfang; Liu Jianxin; Lin Yangyang; Lou Jiwu;
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正文语种 eng
中图分类诊断学;
关键词
Melting curve; Dually labelled probes; alpha-Thalassaemia; Deletion;

机译：熔解曲线;双标记探针;α-地中海贫血;缺失;

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1. Rapid detection of alpha-thalassaemia alleles of (_SEA)/, -alpha(3.7)/ and -alpha(4.2)/ using a dual labelling, self-quenching hybridization probe/melting curve analysis [J] . Gao Lan, Liu Yanhui, Sun Manna, Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines . 2015,第6期

机译：使用双重标记，自猝灭杂交探针/熔解曲线分析快速检测（_SEA）/，-α（3.7）/和-alpha（4.2）/的α地中海贫血等位基因
2. Rapid detection of Mmalton alpha(1)-antitrypsin deficiency allele by real-time PCR and melting curves in whole blood, serum and dried blood spot samples [J] . Belmonte Irene, Montoto Luciana, Miravitlles Marc, Clinical chemistry and laboratory medicine: CCLM . 2016,第2期

机译：通过实时PCR和全血，血清和干血斑样品中的熔解曲线实时检测Mmalton alpha（1）-抗胰蛋白酶缺乏等位基因
3. DETECTION OF DELETION alpha(+)-THALASSEMIA MUTATION [-alpha (3.7), -alpha (4.2)] BY QUANTITATIVE PCR ASSAY [J] . Seeratanachot Teerapat, Shimbhu Dawan, Charoenkwan Pimlak, The Southeast Asian journal of tropical medicine and public health . 2015,第Pta1期

机译：通过定量PCR分析检测甲型（+）-地中海贫血突变[-α（3.7），-α（4.2）]
4. Identification of squid species by melting temperature shifts on fluorescence melting curve analysis (FMCA) using single dual-labeled probe [C] . Eunjung Koh, Ha Jeong Song, Na Young Kwon, Smart sensors, actuators, and MEMS VIII . 2017

机译：使用单个双标记探针通过荧光解链曲线分析（FMCA）上的解链温度变化鉴定鱿鱼种类
5. Multiplex Fluorescence Melting Curve Analysis for Mutation Detection with Dual-Labeled Self-Quenched Probes [O] . Qiuying Huang, Zanzan Liu, Yiqun Liao, 2011

机译：多重荧光熔解曲线分析突变检测与双重标记自淬火探针
6. Pesquisa das deleções -α3.7 e -α4.2 no agrupamento de genes da alfa globina humana e avaliação do perfil clínico-laboratorial em indivíduos com fenótipo de hemoglobina AS Study of the -α3.7 and -α4.2 deletions in the group of genes of human alpha globin and evaluation of the clinical-laboratory profile in individuals with the hemoglobin AS phenotype [O] . Tatiana L. S. Nogueira 2009

机译：人类α珠蛋白基因簇中-α3.7和-α4.2缺失的研究以及血红蛋白AS表型个体的临床和实验室概况评估该组中-α3.7和-α4.2缺失的研究血红蛋白AS表型的人α珠蛋白基因的鉴定和临床实验室概况的评估

Rapid detection of alpha-thalassaemia alleles of (_SEA)/, -alpha(3.7)/ and -alpha(4.2)/ using a dual labelling, self-quenching hybridization probe/melting curve analysis

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