Effects of crude extracted proteins from supernatant of BCF-P and EGF on acid secretion

摘要

In order to compare the in vitro effects of crude extracts of broth culture filtrate protein (BCF-P) from the supernatant of an H. pylori liquid culture and epidermal growth factor (EGF) on acid secretion by isolated rabbit gastric parietal cells. Isolated rabbit gastric parietal cells were incubated with BCF-P or EGF for 1 and 12 h. Morphological changes were assessed by electron microscopy, and C-14-aminopyrine (C-14-AP) uptake and H+-K(+)ATP mRNA expression were measured. At 50 mu g/mL, BCF-P stimulated acid secretion by parietal cells (P < 0.05) at 1 h but not at 12 h. At 100 mu g/mL, BCF-P significantly inhibited histamine-stimulated acid secretion (P < 0.05) and decreased H+- K(+)ATP mRNA expression in parietal cells, but it was reversed after EGF exposure. Crude extracted proteins from BCF-P damaged the inner cell membrane structure of parietal cells, reduced H+-K(+)ATP gene expression, and inhibited acid secretion, activities that differed markedly from those of EGF.