Combinatorial Libraries of Phage Antibodies: Application for Analysis of Gene Segments Encoding Variable Domains of Autoantibodies to Tumor Necrosis Factor

摘要

Twenty unique phage antibodies to human tumor necrosis factor alpha were selected from a naive combinatorial library of human single chain variable fragments. Analysis of gene segments encoding selected antibodies showed that the repertoire of variable domains of heavy and light chains included variable domains of both naive autoantibodies and antibodies produced through somatic hypermutation.