Polymorphism in mtDNA control region of Mizo-Mongloid Breast Cancer samples as revealed by PCR-RFLP analysis

摘要

Mutations in mitochondrial D-loop region of DNA (mtDNA) may serve as a potential sensor for cellular DNA damage and marker for cancer development. We investigated the restriction fragment length polymorphism (RFLP) pattern of the D-loop region in the blood samples of breast cancer patients among Mizoram population. Significant differences were observed among breast cancer and healthy blood samples in the RFLP pattern using Alul, Haelll and Rsal enzymes. Polymorphic information content (PIC - 0.258), band informativeness (, - 3.283) and marker index (Ml - 0.006) were highest in the case of Rsal enzyme. Our data suggest that the Rsal polymorphic site in the mitochondrial control region is an informative marker for breast cancer development in Mizo population.