首页> 外文期刊>Mitochondrial DNA, Part A >Sequencing and analysis of the complete mitochondrial genome Aedes albopictus (Diptera: Culicidae) in China
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Sequencing and analysis of the complete mitochondrial genome Aedes albopictus (Diptera: Culicidae) in China

机译:中国线粒体完整基因组白纹伊蚊(双翅目:葫芦科)的测序与分析

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摘要

The complete sequence of the mitochondrial genome of the Aedes albopictus (Diptera: Culicidae) is presented using traditional Sanger sequencing. Its mitogenome are 16,660 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes,2 ribosomal RNA (rRNA) genes and a non-coding A + T rich region. As in other insects, most mitochondrial genes are encoded on the heavy strand, except for ND5, ND4, ND4L, ND1, two rRNA and eight tRNA genes, which are encoded on the light strand. The overall base composition on heavy strand was as follows - A: 40.1 %, G: 8.2%, C: 11.9%, T: 39.8% and the A + T content 79.9%. The results of phylogenetic analyzes showed that theAe. albopictus has closed relationship with the family Culicidae and order Diptera.
机译:使用传统的Sanger测序方法,可以得到白纹伊蚊(Diptera:Culicidae)线粒体基因组的完整序列。它的有丝分裂基因组长16660 bp,由13个蛋白质编码基因,22个转移RNA(tRNA)基因,2个核糖体RNA(rRNA)基因和一个非编码A + T富集区域组成。与其他昆虫一样,大多数线粒体基因都在重链上编码,除了ND5,ND4,ND4L,ND1,两个rRNA和八个tRNA基因都编码在轻链上。重链的总碱组成如下:A:40.1%,G:8.2%,C:11.9%,T:39.8%,A + T含量为79.9%。系统发育分析结果表明,Ae。 albopictus与葫芦科和双翅目有密切的联系。

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