Microscopy beyond the resolution limit: Photoactivated localization microscopy (PALM) [Mikroskopie jenseits der aufl?sungsgrenze: Photoactivated localization microscopy (palm)]

摘要

Super Resolution Microscopy Resolution in conventional light microscopy is restricted by the borders of physics. The limiting factor is the wavelength of the used light. Nevertheless, many essential intracellular components such as the cytoskeleton or secretory vesicles are in size well below this diffraction limit. Super Resolution Microscopy techniques such as Photoactivated Localization Microscopy make it possible to break through this diffraction barrier. Controlled activation and sampling of a subpopulation of photoactivatable fluorophores enables localization of probes in a nanometric scale.