Preoperative seminal plasma disturbed oxidant/antioxidant milieu could predict failure of varicocelectomy as a therapeutic modality for male infertility

摘要

The aim of this study was to evaluate seminal plasma (SP) malonaldehyde (MDA), superoxide des-mutase (SOD) and CoQ10 levels in infertile men with and without varicocele as a trial to evaluate the pre-dictability of their preoperative estimation for postop-erative (PO) improvement of seminal parameters. Methods. Seventy infertile men; with (N.=35) and with-out (N.=35) and 20 fertile men with (N.=l 0) and without (N.=10) clinically and ultrasonographically diagnosed varicocele. Infertile men with varicocele were assigned to undergo bilateral varicocelectomy using subinguinal approach. All infertile patients had preoperative hor-monal profile. Semen samples were obtained from all men for standard semen analysis and SP estimation of CoQ10 using high-performance liquid chromatography (HPLC), SP MDA using a thiobarbituric acid reactive substances (TBARS) assay and SP SOD activity by che-miluminescence. Another semen sample was obtained PO for standard semen analysis.