New Structures of the O-Specific Polysaccharides of Proteus.4.Polysaccharidels Containing Unusual Acidic N-Acyl Derivatives of 4-Amino-4,6-dideoxy-D-glucose

摘要

The structures of the O-polysaccharides of the lipopolysaccharides of Proteus mirabilis O7 and O49 were determined by chemical methods,mass spectrometry,including MS/MS,and NMR spectroscopy,including experiments run in an H_2O/D_2O mixture to reveal correlations for NH protons.The O-polysaccharides were found to contain N-carboxyacetyl(malonyl)and N-(3-carboxypropanoyl)(succinyl)derivatives of 4-amino-4,6-dideoxyglucose(4-amino-4-deoxyquinovose,Qui4N),respectively.The behavior of Qui4N derivatives with the dicarboxylic acids under conditions of acid hydrolysis and methanolysis was studied using GLC-MS.