Phorbol Ester Stimulates Expression of the Human Tryptophanyl-tRNA Synthetase Gene

摘要

The effect of the phorbol ester phorbol 12-myristate 13-acetate (PMA) on expression of the human interferon (IFN)-inducible tryptophanyl-tRNA synthetase (WRS) gene was studied.PMS caused an increase in the basal and IFN-gamma-induced WRS protein content in HeLa and HEK293 cultured cells.Besides,PMA upregulated WRS mRNA level in HeLa cells.Since PMA is known as a selective activator of proteinkinase C(PKC) and is widely used to study the PKC-related pathwaysthese results show possible PKC involvement in regulation of the WRS gene expression.PKC inhibition by stau-rosporine 910 and 100 nM) had no effect on either basal or IFN-gamma-induced expression of WRS in either cell line.Consequently,PKC is not an indispensable element in WRS induction by IFNgamma.Rather,PKC may activate WRS gene expression only by a distinct pathway.