Influence of pH on low temperature inactivation of the extracellular proteinase from Pseudomonas fluorescens 22F.

摘要

The effect of pH on inactivation of the extracellular proteinase from Pseudomonas fluorescens 22F at 35-70deg C was studied. At 50deg C the proteinase was most stable at pH 6 and 10, and least stable at pH 8. Between pH 6 and 10, inactivation was probablycaused by autoproteolysis. The denaturation temperature was dependent on pH. The proteinase was more stable at pH 5.8 than at pH 7.0, 7.5 and 9.6.