Using Scientific CMOS Technology for Fast 3D Imaging with Selective-Plane Illumination

摘要

A major challenge at the interface between cell biology and developmental biology is to connect the dynamics of cellular processes with large-scale morphological changes at the organism level. Selective-plane illumination microscopy (SPIM) has recently attracted a lot of attention, in particular for whole animal imaging. Here we show that the modern CMOS technology based cameras can yield unprecedented imaging speeds in combination with light-sheet illumination. Using a light-sheet based microscope that consisted of two illumination and one detection arms, we demonstrate the high performance of the sCMOS camera by imaging cell membranes in the early embryo of Drosophila melanogaster.