Serum Calprotectin as a Novel Biomarker in Abdominal Aortic Aneurysm Pathogenesis and Progression: Preliminary Data from Experimental Model in Rats

摘要

Background: Abdominal aortic aneurysm (AAA) formation is associated with by inflammation and matrix degradation. This study tested the hypothesis that calprotectin, a novel biomarker for inflammation, as well as established biomarkers such as C-reactive protein (CRP) and matrix metalloproteinase-9 (MMP-9) could also be indicative inflammatory biomarkers during AAA pathogenesis and progression. We also evaluated the correlation of serum soluble Receptor for Advanced Glycation End Products (sRAGE) with AAA diameter and serum calprotectin levels. Materials and Methods: Rat abdominalaortas were perfused with porcine pancreatic elastase (AAA Group) or saline (Control Group) and studied on post-perfusion days 7 and 14 (n=11 per treatment group). Aneurysm was defined as a dilatation of the aorta above 150 of its original diameter. Laparotomy was performed on days 0 (T0),7 (T7) and 14 (T14) for aortic diameter measurement. At the same time intervals, we measured the serum levels of calprotectin, CRP, sRAGE and MMP-9. Results: All animals developed AAA and no rupture occurred. MMP-9 in AAA group at T14 (pConclusion: This is the firstevaluation of calprotectin as an AAA inflammation biomarker. It seems to be a promising marker related to AAA natural history. Further experimental and large human studies are needed to fully elucidate the role of calprotectin in the development and progression of AAAs.