Determination of atrazine,desethyl atrazine and desisopropyl atrazine in environmental water samples using hollow fiber-protected liquid-phase microextraction and high performance liquid chromatography

摘要

A new method based on hollow fiber-protected liquid-phase microextraction(LPME) was developed for the simultaneous determination of at razine,desethyl atrazine and desisopropyl atrazine in environmental water samples.In LPME,analytes were extracted into 1-octanol immobilized in the micropores of a poly(vinylidene fluoride) porous hollow fiber membrane,and back extracted into the acceptor(4 M HCl) filled in the lumen of the hollow fiber.After LPME,the analytes trapped in the acceptor were analyzed with high-performance liq uid chromatography after neutralization.The effect of extraction factors such as sample pH,acceptor pH,salinity,extraction time,stirring rate,and humic acid were studied.Under the optimized conditions,the limits of detection and relative standard deviations were respectively in the range of 0.5-1.0 mu g L-1 and 3.9-4.7%(n=5).The proposed method was applied to determine atrazine,desethyl atrazine and desisopropyl atrazine in wastewater and ground water samples.The three analytes were below the limits of detection,but good relative spiked recov eries over 90.1+-5.9% at 5 mu g L-1 spiked level were obtained.