Hydrophilic ionic liquids as reaction media for the determination of guaiacol using horseradish and soybean peroxidases

摘要

The advantages of hydrophilic ionic liquids - 1-butyl-2-methylimidazolium and N-butyl-3-methylpyridinium tetrafluoroborates - over conventional polar solvents (DMSO and acetonitrile) for the enzymatic determination of 0.05-3 mm guaiacol in the reaction media containing 20-40 vol% H_2O are shown as the result of a comparative study of the kinetics of guaiacol oxidation by ButOOH catalyzed by native horseradish and soybean peroxidases.