Solid phase microextraction for the determination of volatile organics in the foliage Fraser fir (Abies fraseri)

摘要

Solid phase micro extraction(SPME) was investigated for the determination of volatile chemicals in the foliage of Fraser fir (Abies fraseri).Advantages of SPME include its speed,sensitivity,and ability to determine volatiles wighout chemical solvents.In this study,qualitative comparisons were performed of the volatiles emitted from living foliage (in situ),damaged living foliage (in situ damaged),and severed foliage (ex situ).These studies suggest that #beta#-phellandrene and #gamma#-terpinene may be emitted as a respinse to foliar injury.Comparisons were made of the volatiles collected by SPME with ex situ sampling to previous studies in our laboratory using methylene chloride extraction.Similar results were obtained by the two extraction methods.