Peculiarities in the chromosomal DNA structure in Sulfobacillus thermosulfidooxidans analyzed by pulsed-field gel electrophoresis

摘要

A polymorphism of the chromosomal DNA structure in Sulfobacillus thermosulfidooxidans cultures was studied by pulsed-field gel electrophoresis. The structure of DNA from the type strain VKM B-1269 remained stable regardless of the energy source (yeast extract, Fe2+, S-0, sulfide minerals, ore concentrate) used by the culture, whereas changes in the genome of the non-spore-forming strain S. thermosulfidooxidans subsp. asporogenes 41 were revealed upon switching the culture from the oxidation of yeast extract to the oxidation of elemental sulfur. A microbial culture, outgrowing strain VKM B-1269 under certain conditions, capable of oxidizing Fe2+, and possessing a NotI-restriction pattern of chromosomal DNA distinct from those of the known S. thermosulfidooxidans cultures, was isolated during continuous cultivation under non-sterile conditions in laboratory apparatus and during semi-industrial leaching of gold from an ore concentrate obtained from the Nezhdaninskoe deposit. [References: 11]