Effect of eggs desticking using protease proteolytic enzyme and pineapple juice in the larviculture of the puffer fish Sphoeroides annulatus

摘要

Recent findings show that eliminating the gelatinous egg capsule surrounding the fertilized eggs of the puffer fish Sphoeroides annulatus improves embryonic development and hatching rate. In this work, the following 3 different desticking procedures were used to evaluate larval growth and survival: 5 ml L-1 proteolytic protease enzyme (EPP), 1 of pineapple juice (JP) and eggs with no sticky coating on the surface as a control (C). All treatments were carried out in triplicate and the experiment lasted for 33 days. The fertilization percentage obtained was 98.6 +/- 1.8 in all treatments. The highest hatching percentage was achieved with EPP (93.03 +/- 3.01) and the lowest with JP (80.97 +/- 3.97). Both total length (LT) (14.4 +/- 0.40 mm) and weight (P) (0.118 +/- 0.004 g) were significantly higher in the JP treatment. The highest survival rate was obtained with EPP (19.34 +/- 1.98) and differences were found to be statistically significant. The desticking of eggs treated with JP gave a low hatching percentage due to the fruits pH reading of 4 and since fewer organisms were produced, higher growth rate was obtained. Thus, we conclude that removing the adhesive layer of the egg does not affect larval growth and survival.