Extraction of DNA from paraffin sections with proteinase K and DNAzol.

摘要

Currently, the use of phenol/chloroform is the method of choice for DNA isolation from paraffin sections. However, in addition to its toxicity, the phenol/chloroform process is time-consuming and DNA can be lost. In the present study an alternative method is described which uses proteinase K and DNAzol for DNA extraction from paraffin-embedded tissue that is simple, quick and efficient.