Visualizing estrogen receptor-alpha-expressing neurons using a new ER alpha-ZsGreen reporter mouse line

摘要

Background. A variety of biological functions of estrogens, including regulation of energy metabolism, are mediated by neurons expressing estrogen receptor-alpha (ER alpha) in the brain. However, complex intracellular processes in these ER alpha-expressing neurons are difficult to unravel, due to the lack of strategy to visualize ER alpha-expressing neurons, especially in unfixed brain tissues.