CYTOTOXICITY OF ACULEATISDM-A TO.. HEPATOCELLULAR CARCINOMA CELLS DETERMINED BY MTT BIOASSAY

摘要

From the methanolic extract of fresh fruits of Solarium reflexum Schrank, a major compound was isolated and identified as; aculeatiside-A 1. The structure of the isolated compound was elucidated on the basis of ~1H and ~(13)C and DEPT-~(13)C-NMR; in addition to comparison with reported data. The cytotoxic effect of aculeatiside-A on hepatocellular carcinoma cells and its mechanism was investigated. It was accomplished that aculeatiside-A inhibit the growth of HepG-2 cells significantly by induction of apoptosis. Marked morphological changes of apoptosis were observed clearly. The results for the first time demonstrate that aculeatiside-A has significant anti-proliferation effect by induction of apoptosis on hepatocellular carcinoma cells in-vitro, down regulation of Survivin and Bcl-2 expressions as well as up regulation of Box and P~(53) expression may be one of the important apoptotic inducing mechanisms.