Association between body weight and age of dogs and global DNA methylation

摘要

The aim of the study was to analyze whether the total DNA methylation level in dogs is dependent on age and body weight, using global DNA methylation techniques modeled on the ELISA assay. The material for the study consisted of blood collected from dogs of varying age and body weight. The experimental group consisted of 16 individuals with body weights ranging from 3.2 kg to 70 kg and aged from 2 months to 14 years. Total DNA methylation was determined using a kit for quantitative analysis of DNA methylation manufactured by MDQI, Imprint Methylated DNA Quantification Kit (Sigma Aldrich). The total DNA methylation level in the dog genome increases with age. The technique of analyzing the DNA methylation level based on the ELISA enzyme immune assay is an efficient and effective method for determining relative DNA methylation level.