Effect of cadmium on the activity of antioxidant enzymes in isolated rat hepatocytes.

摘要

The aim of the study is to evaluate the effect of cadmium on the antioxidant enzyme activity in rat hepatocytes. The experiments were conducted on isolated rat hepatocytes, which were incubated two hours in modified Williams' E medium (MWE) with 25, 50, and 200 micro M cadmium chloride (CdCl₂). Hepatocytes incubated in MWE medium without cadmium chloride were used as a control. The activity of antioxidant enzymes - superoxide dismutase (SOD1 and SOD2), catalase (CAT), total glutathione peroxidase (tot. GSHPx), selenium-dependent glutathione peroxidase (Se-GSHPx) and glutathione reductase (GSHR) - and the values of the studied oxidative stress markers - the concentration on of thiobarbituric-acid reacting substances (TBARS) and reduced glutathione (GSH)- indicate that cadmium induces oxidative stress in rat hepatocytes which impairs antioxidative mechanisms.