Dot-blot for the diagnosis of bovine spongiform encephalopathy.

摘要

The article presents the practical use of dot-blot for the diagnosis of BSE. This method enables obtaining test results in a short period. The practical implementation of this method for the diagnosis of BSE is reinforced by the simple test procedure, low equipment requirements and low volume of waste produced. All samples from twenty eight confirmed Polish cases of BSE had values above negative control samples. Only one positive sample had an optical density close to negative samples. This sample came from an atypical case of BSE, which in active monitoring with the rapid test had values slightly above the cut off. All remaining samples from positive cases had values above the mean for negative samples plus two standard deviations..