Applying macro-restriction analysis in differentiating selected strains of the Bacillus cereus group.

摘要

The aim of this study is to apply macro-restriction analysis in order to differentiate selected strains of the Bacillus cereus group. Three different restriction enzymes (SfiI, NotI and SmaI) were used during the research process and it was confirmed that SmaI is the best restriction enzyme applied in macrorestriction analysis of the Bacillus cereus group. The study used 89 Bacillus cereus group strains, and an identical restriction pattern generated by restriction enzymes was confirmed among 23 of the studied Bacillus anthracis strains. Two groups of strains (four and two strains) among 19 Bacillus sp. Ba 813 and three groups (three, two and two strains) among 44 strains of Bacillus thuringiensis showed identical restriction profiles. The similarity of studied strains was ascertained on the basis of the Dice coefficient, and confirmed that strains showing the same restriction profiles were identical (100% similarity). The similarity between the remaining strains of the Bacillus cereus group and the Bacillus anthracis strains oscillated between 20% to 78.6%, while the similarity of these strains to each other oscillated from 15.4% to 100%. Dendrograms were also constructed using the UPGMA method..