Evaluation of lymphocyte subpopulations, phagocytic activity and oxygen metabolism of granulocytes of rabbits infected with Trichophyton mentagrophytes and immunized against trichophytosis.

摘要

The aim of the study was to evaluate the CD3, CD4, CD8, CD19, CD25 T lymphocyte subpopulations and the state of granulocytes activity in the peripheral blood of rabbits infected with Trichophyton mentagrophytes and immunized against trichophytosis using flow cytometry. Our study revealed significant suppression of non-specific cellular antimycotic immunity in rabbits during the development of fungal lesions, which manifested a significant decrease in the phagocytic activity and oxygen metabolism of granulocytes and the decrease in CD3, CD4 subpopulations and CD25 T lymphocytes. Simultaneously, the increase of suppressor CD8 T cells and CD4/CD8 T-cells ratio were observed. The vaccine Alopevac can be used as an effective vaccine against rabbit trichophytosis. Alopevac restored non-specific cellular antimycotic immunity and proper CD4/CD8 T-cells ratio, which contributed to the effective elimination of fungal lesions.