Studies on cytotoxity of monensin and narasin in rat hepatocyte cell line culture.

摘要

The aim of the work was to determine monensin, narasin hepatotoxicity and the nature of cell death. Rat hepatocyte model cell line (FAO) was used to investigate two ionophore antibiotic cytotoxic effects estimated by MTT, NRU and KB tests approved by INVITTOX. Additionally, the apoptoticecrotic nature of cell death was determined by propiodine iodide and HO 342 staining of the cultured hepatocytes. IC50 indices for monensin and narasin estimated by using the MTT test during a 24-h incubation period were at a level of 0.027+or-0.001 and 0.037+or-0.001 micro M, respectively. However, an incubation period of 48 h yielded an equal value (-0.02 micro M) for both ionophores. Contrary to the MTT test, NRU and KB estimations demonstrated lower IC50 values for narasin than for monensin. These results correlated to in vivo acute toxicity and LD50 indices in rats (data from references). The apoptotic nature of hepatocyte death dominated in the cultures. The mechanisms of ionophore induced cytotoxicity are also discussed..