Occurrence of Mycobacterium paratuberculosis in samples of raw milk.

摘要

The aim of this study was to determine the frequency of Mycobacterium paratuberculosis ([M. avium subsp. paratuberculosis], MAP) occurrence in raw milk samples from northeastern Poland. Samples of udder milk were collected from herds in which seropositive reactions on paratuberculosis had been confirmed in samples of bulk tank milk from production farms. Standardization and decontamination of milk samples from fast-growing microflora was carried out prior to research, which was performed by using the culture method. PCR technique was used to distinguish MAP from other Mycobacterium, thus enabling the IS-900 fragment to be detected. The presence of MAP was confirmed in 2.4% of udder milk samples from northeastern Poland, but none was confirmed in bulk tank milk samples from this region..