Monkey brain as a model for Alzheimer's pathology

摘要

The aim of the studies was to determine the dynamics and progress of amyloidosis-β and neurofibrillary tangles (NFT) in various 7 to 33-year-old monkeys. Tissue samples from the brains were fixed in 10% neutral formalin and embedded in paraffin by aroutine procedure. Sections 7 m were stained with hematoxylin and eosin, Nissl, Congo red, Bielschowsky, Tioflavin S, and monoclonal antibodies (4G8 raAb). Neuroflbrillary tangles were stained with anti-TAU monoclonal antibody. Senile plaques were revealed in six of 11 monkeys. All three types of plaque were present, but the primitive form was strongly predominant Neuritic plaques (primitive and classical) were seen in frontal, temporal, parietal and occipital cortices. Particularly diffuse types of plaque were found in a Lemur mongoz brain. Congofflic angiopathy (CAA) was observed in meningeal and cortical vessels of six monkeys above 19-years-of-age. Neurofibrillary tangles (NFT) and neurofibrillary threads were observed in dendrites and axons of CA1neurons, CA3 hippocampal formation and temporal cortex in two monkeys only with the mAb Tau-1 method (Cebus apella, aged 23, and Lemur mongoz, 22-years-old). The study revealed that brain aging in monkeys is marked by an increasing number of preamyloiddeposits in the neuropil and by CAA of the leptomeningeal and parenchymal vessels.