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Applying the microarray (chip) DNA technique for bacteriological examinations of foodstuffs [Polish]

机译:将微阵列(芯片)DNA技术应用于食品的细菌学检查[波兰语]

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摘要

Conventional methods used in bacteriological examinations of foodstuffs are time-consuming and do not always result in the correct identification of the microorganism. For this reason laboratories more and more frequently employ highly sensitive, molecular techniques. One of them is the microarray DNA method. The aim of the study was to check the use of NUTRI(R)-Chip (GeneScan Europe AG, Germany) for the detection of the following bacteria: Campylobacter coli, Campylobacter was affirmed in four cases and Listeria monocytogenes in one case. Thanks to the existence of control spots on the chip, it was possible to avoid adulterating results. Moreover, microarray technique made it possible to obtain the results of the study in a relatively short period of time.
机译:食品细菌学检查中使用的常规方法很耗时,并不总能正确鉴定微生物。因此,实验室越来越多地采用高度敏感的分子技术。其中之一是微阵列DNA方法。该研究的目的是检查使用NUTRI-Chip(德国GeneScan欧洲公司)检测以下细菌的情况:大肠杆菌弯曲杆菌,确认弯曲杆菌4例和单核细胞增生李斯特氏菌1例。由于芯片上存在控制点,因此可以避免掺假结果。此外,微阵列技术使在相对较短的时间内获得研究结果成为可能。

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